کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5764124 | 1625913 | 2017 | 45 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cellular uptake and intracellular localization of poly (acrylic acid) nanoparticles in a rainbow trout (Oncorhynchus mykiss) gill epithelial cell line, RTgill-W1
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کلمات کلیدی
CTBddH2OMacropinocytosis4′ 6-diamidino-2-phenylindoleGTPaseAnnexin V-FITCCPZCaveolae-mediated endocytosisDAPIPBS/BSATransferrinRTgill-W1PBSDEXFBSDMSO - DMSOdouble-distilled water - آب دوتاییCME - آموزش مداومEndocytosis - آندوسیتوز یا درون بریClathrin-mediated endocytosis - اندوسیتوز مداخله می کند ClathrinInternalization - داخلی سازیDextran - دکسترانDimethyl sulfoxide - دیمتیل سولفواکسیدEx/Em - سابق / منfetal bovine serum - سرم جنین گاوPhosphate buffered saline - فسفات بافر شورLysosome - لیزوزومGenistein - ماده جنستئینFish - ماهیNanotoxicology - نانوتوکسیکولوژی، سم شناسی نانوNanoparticle - نانوذارتNile Red - نیل قرمزwortmannin - ورتمنینchlorpromazine - کلرپرمازینcholera toxin B subunit - کلسترول توکسین B واحدColocalization - کلوکالیزاسیونguanosine triphosphatase - گوانوزین تری فسفاتاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The ever-growing production of engineered nanoparticles (NPs) for use in many agricultural, commercial, consumer, and industrial applications will lead to their accidental or intentional release into the environment. Potential routes of environmental exposure include manufacturing or transport spills, disposal of NPâcontaining products down the drain and/or in landfills, as well as direct usage on agricultural land. Therefore, NPs will inevitably contaminate aquatic environments and interact with resident organisms. However, there is limited information regarding the mechanisms that regulate NP transport into fish from the environment. Thus, our primary objective was to elucidate the mechanism(s) underlying cellular uptake and intracellular fate of 3-9Â nm poly (acrylic acid) NPs loaded with the fluorescent dye Nile red using a rainbow trout (Oncorhynchus mykiss) gill epithelial cell line (RTgillâW1). In vitro measurements with NPâtreated RTgill-W1 cells were carried out using a combination of laser scanning confocal microscopy, flow cytometry, fluorescent biomarkers (transferrin, cholera toxin B subunit, and dextran), endocytosis inhibitors (chlorpromazine, genistein, and wortmannin), and stains (4â², 6âdiamidino-2-phenylindole, Hoechst 33342, CellMask Deep Red, and LysoTracker Yellow). Clathrin-mediated endocytosis (CME), caveolaeâmediated endocytosis and macropinocytosis pathways were active in RTgillâW1 cells, and these pathways were exploited by the non-cytotoxic NPs to enter these cells. We have demonstrated that NP uptake by RTgillâW1 cells was impeded when clathrin-coated pit formation was blocked by chlorpromazine. Furthermore, colocalization analysis revealed a moderate positive relationship between NPs and LysoTracker Yellow-positive lysosomal compartments indicating that CME was the dominant operative mechanism involved in NP internalization by RTgill-W1 cells. Overall, our results clearly show that fish gill epithelial cells internalized NPs via energyâdependent endocytotic processes. This study enhances our understanding of complex NPâcell interactions and the results obtained in vitro imply a potential risk to aquatic organisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 192, November 2017, Pages 58-68
Journal: Aquatic Toxicology - Volume 192, November 2017, Pages 58-68
نویسندگان
Lindsey C. Felix, Van A. Ortega, Greg G. Goss,