کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5764328 | 1625919 | 2017 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of Florida Red Tides on histone variant expression and DNA methylation in the Eastern oyster Crassostrea virginica
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کلمات کلیدی
NTCFRTNSPBrevetoxinsHABsPTMsMSAPAMOVAPCA - PCASDS-PAGE - الکتروفورز ژل پلی آکریل آمیدHistone variants - انواع هیستونEpigenetics - اپی ژنتیکNo template control - بدون کنترل قالبanalysis of molecular variance - تجزیه و تحلیل واریانس مولکولیPrincipal component analysis - تحلیل مولفههای اصلی یا PCApost-translational modifications - تغییرات پس از ترجمه، پیرایش پساترجمهFlorida red tide - جغرافیای قرمز فلوریداharmful algal blooms - شکوفه های جلبک مضرOysters - صدفDNA methylation - متیلاسیون DNAEnvironment - محیط زیستneurotoxic shellfish poisoning - مسمومیت با سموم روغنی عصبی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effects of Florida Red Tides on histone variant expression and DNA methylation in the Eastern oyster Crassostrea virginica Effects of Florida Red Tides on histone variant expression and DNA methylation in the Eastern oyster Crassostrea virginica](/preview/png/5764328.png)
چکیده انگلیسی
Massive algal proliferations known as Harmful Algal Blooms (HABs) represent one of the most important threats to coastal areas. Among them, the so-called Florida Red Tides (FRTs, caused by blooms of the dinoflagellate Karenia brevis and associated brevetoxins) are particularly detrimental in the southeastern U.S., causing high mortality rates and annual losses in excess of $40 million. The ability of marine organisms to cope with environmental stressors (including those produced during HABs) is influenced by genetic and epigenetic mechanisms, the latter resulting in phenotypic changes caused by heritable modifications in gene expression, without involving changes in the genetic (DNA) sequence. Yet, studies examining cause-effect relationships between environmental stressors, specific epigenetic mechanisms and subsequent responses are still lacking. The present work contributes to increase this knowledge by investigating the effects of Florida Red Tides on two types of mechanisms participating in the epigenetic memory of Eastern oysters: histone variants and DNA methylation. For that purpose, a HAB simulation was conducted in laboratory conditions, exposing oysters to increasing concentrations of K. brevis. The obtained results revealed, for the first time, the existence of H2A.X, H2A.Z and macroH2A genes in this organism, encoding histone variants potentially involved in the maintenance of genome integrity during responses to the genotoxic effect of brevetoxins. Additionally, an increase in H2A.X phosphorylation (γH2A.X, a marker of DNA damage) and a decrease in global DNA methylation were observed as the HAB simulation progressed. Overall, the present work provides a basis to better understand how epigenetic mechanisms participate in responses to environmental stress in marine invertebrates, opening new avenues to incorporate environmental epigenetics approaches into management and conservation programs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 186, May 2017, Pages 196-204
Journal: Aquatic Toxicology - Volume 186, May 2017, Pages 196-204
نویسندگان
Rodrigo Gonzalez-Romero, Victoria Suarez-Ulloa, Javier Rodriguez-Casariego, Daniel Garcia-Souto, Gabriel Diaz, Abraham Smith, Juan Jose Pasantes, Gary Rand, Jose M. Eirin-Lopez,