The rhoptry proteome of Eimeria tenella sporozoites

► Nine independent preparations of Eimeria tenella sporozoite rhoptries were analysed. ► Mass spectrometry was carried out on 1-DE and 2-DE separated proteins. ► 347 targets were identified including orthologues of known RONs and ROPs and novel proteins. ► Many of the RON proteins were differentially expressed between parasite stages. ► Two ROP kinases were found in the sporozoite rhoptry proteome.