کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5794442 | 1554304 | 2016 | 4 صفحه PDF | دانلود رایگان |
- The pathogenicity of Toxoplasma gondii from two Brazilian genotypes is compared.
- Experimental infections are performed on mice model inoculated orally with oocysts.
- Mortality rate of isolates from genotype BrI is not dose dependant.
- Virulence may be stage dependant for genotype BrIII.
- An intra-genotype variation of pathogenicity is demonstrated among BrIII isolates.
South American strains of Toxoplasma gondii present higher genetic diversity than classical European strains. We compared the virulence of two non-archetypal Brazilian genotypes of T. gondii to mice. Oocysts of four isolates, two genotype BrI (TgCatBr71 and TgShBr11) and two BrIII (TgCatBr74 and TgCatBr60) were obtained from cats fed experimentally infected mice. After sporulation, 5.0Â ÃÂ 101 and 1.0Â ÃÂ 102 oocysts were orally administrated to Swiss albine mice in Experiments #1 and #2, respectively (4-10 mice/group). Humoral response from dead and surviving mice was analyzed on days 9 to 35 post-infection. Microscopic observations of lungs and brains were performed for tachyzoites and cysts visualization in fresh preparations. Negative results were tested by PCR. Virulence after infection with oocysts is dose dependent for genotype BrIII isolates, but not for BrI. Differences in mortality were observed among isolates from genotype BrIII on Experiment #1. Intra-genotype phenotypic variation related to the parasite stage of infection was demonstrated and this characteristic should be further studied and may influence future work regarding the role of virulence amid hosts.
Journal: Research in Veterinary Science - Volume 107, August 2016, Pages 257-260