کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5802016 1555650 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Vaccine potential of recombinant cathepsinL1G against Fasciola gigantica in mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Vaccine potential of recombinant cathepsinL1G against Fasciola gigantica in mice
چکیده انگلیسی


- Recombinant mature CathesinL1G (rmFgCatL1G) exhibited vaccine potential against F. gigantica infection in mice.
- Vaccination with rmFgCatL1G showed high levels of protection at 56.5-58.3%.
- Vaccination with rmFgCatL1G reduced the pathological lesions of livers.
- The worms from vaccinated group were generally decreased in weight.
- FgCatL1G protein was expressed at high level in caecal epithelium of F. gigantica.

In this study, we characterized and investigated the vaccine potential of FgCatL1G against Fasciola gigantica infection in mice. Recombinant mature FgCatL1G (rmFgCatL1G) was expressed in Escherichia coli BL21. The vaccination was performed in Imprinting Control Region (ICR) mice (n = 10) by subcutaneous injection with 50 μg of rmFgCatL1G combined with Freund's adjuvant. Two weeks after the second boost, mice were infected with 15 metacercariae by the oral route. The percents of protection of rmFgCatL1G vaccine were estimated to be 56.5% and 58.3% when compared with non vaccinated-infected and adjuvant-infected controls, respectively. Antibodies in the immune sera of vaccinated mice were shown by immunoblot to react with the native FgCatL1s in the extract of all stages of parasites and rmFgCatL1H, recombinant pro - FgCatL1 (rpFgCatL1). By immunohistochemistry, the immune sera also reacted with FgCatL1s in the caecal epithelial cells of the parasites. The levels of IgG1 and IgG2a in the immune sera, which are indicative of Th2 and Th1 immune responses, were also increased with IgG1 predominating. The levels of serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) in rmFgCatL1G-immunized group showed no significant difference from the control groups, but pathological lesions of livers in rmFgCatL1G-immunized group showed significant decrease when compared to the control groups. This study indicates that rmFgCatL1G has a vaccine potential against F. gigantica in mice, and this potential will be tested in larger livestock animals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 226, 15 August 2016, Pages 124-131
نویسندگان
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