کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5802266 1555663 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of Trypanosoma vivax using PCR and LAMP during aparasitemic periods
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Detection of Trypanosoma vivax using PCR and LAMP during aparasitemic periods
چکیده انگلیسی


- Capacities of conventional PCR, LAMP and ELISA for detecting T. vivax.
- PCR, LAMP and ELISA were capable of detecting 66.7%, 95.2% and 71.4%, respectively.
- Results may influence the choice of screening tests for cattle herds.

Trypanosoma vivax affects cattle herds in Africa and Americas and has been spreading rapidly in Brazil, through introduction of animals with subclinical infections and without apparent parasitemia, which makes its diagnosis challenging. PCR and LAMP are effective in detecting the presence of T. vivax DNA in situations of low parasitemia. LAMP is simpler and faster technique than PCR, and can be performed in the field, with limited resources. In this study, the capacities of conventional PCR and LAMP for detecting T. vivax in bovine blood samples classified as aparasitemic were evaluated. The capacity of conventional PCR (56.25%) for detecting positive samples was lower than that of LAMP (93.73%). This may influence the choice of screening tests for cattle herds infected with T. vivax.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 214, Issues 1–2, 30 November 2015, Pages 174-177
نویسندگان
, , , , , , ,