The comparison of different ELISA procedures in detecting anti-Trichinella IgG in human infections

The aim of this study was to compare the performance of four different ELISA procedures in diagnosing Trichinella spp. infections in human outbreaks. The main differences between the various procedures were: antigen concentration, dilutions of human serum sample, the type of conjugate used, and time of conjugate incubation. Serum samples were collected from 23 individuals involved in an outbreak. Results obtained in the four tested ELISA procedures differed in the rate of positive results obtained for the examined outbreak. Western blot confirmed the ELISA-positive results. Reactions to the specific 41-45 kDa and 55 kDa bands were obtained with all positive sera.