کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5813649 | 1556619 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Deletion of the Wolfram syndrome-related gene Wfs1 results in increased sensitivity to ethanol in female mice
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کلمات کلیدی
qRT-PCRNMDAN-methyl-d-aspartateLORREthanol - اتانولLoss of righting reflex - از دست دادن رفلکس درست کردنgamma-aminobutyric acid - اسید گاما آمینو بوتیریکGene expression - بیان ژنWolfram syndrome - سندرم ولفرامElevated plus-maze - ماز بعلاوه شکل مرتفعquantitative real-time PCR - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیGABA - گاباGABAA receptor - گیرنده GABAA یا گیرنده گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب رفتاری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Wolfram syndrome, induced by mutation in WFS1 gene, increases risk of developing mood disorders in humans. In mice, Wfs1 deficiency cause higher anxiety-like behaviour and increased response to anxiolytic-like effect of diazepam, a GABAA receptor agonist. As GABAergic system is also target for ethanol, we analysed its anxiolytic-like and sedative properties in Wfs1-deficient mice using elevated plus-maze test and tests measuring locomotor activity and coordination, respectively. Additionally loss of righting reflex test was conducted to study sedative/hypnotic properties of ethanol, ketamine and pentobarbital. To evaluate pharmacokinetics of ethanol in mice enzymatic colour test was used. Finally, gene expression of alpha subunits of GABAA receptors following ethanol treatment was studied by real-time-PCR. Compared to wild-types, Wfs1-deficient mice were more sensitive to ethanol-induced anxiolytic-like effect, but less responsive to impairment of motor coordination. Ethanol and pentobarbital, but not ketamine, caused longer duration of hypnosis in Wfs1-deficient mice. The expression of Gabra2 subunit at 30 minutes after ethanol injection was significantly increased in the frontal cortex of Wfs1-deficient mice as compared to respective vehicle-treated mice. For the temporal lobe, similar change in Gabra2 mRNA occurred at 60 minutes after ethanol treatment in Wfs1-deficient mice. No changes were detected in Gabra1 and Gabra3 mRNA following ethanol treatment. Taken together, increased anxiolytic-like effect of ethanol in Wfs1-deficient mice is probably related to altered Gabra2 gene expression. Increased anti-anxiety effect of GABAA receptor agonists in the present work and earlier studies (Luuk et al., 2009) further suggests importance of Wfs1 gene in the regulation of emotional behaviour.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuropharmacology - Volume 95, August 2015, Pages 59-67
Journal: Neuropharmacology - Volume 95, August 2015, Pages 59-67
نویسندگان
Sirli Raud, Riin Reimets, Maarja Loomets, Silva Sütt, Alina Altpere, Tanel Visnapuu, Jürgen Innos, Hendrik Luuk, Mario Plaas, Vallo Volke, Eero Vasar,