کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5814930 | 1556640 | 2013 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
P2X7 receptors at adult neural progenitor cells of the mouse subventricular zone
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کلمات کلیدی
EGFEmaxPPADS2-MeSATPNPCBrilliant Blue GSVZBBGFGF-2Msi1Bz-ATPTNP-ATPpyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acidEC502-methylthio ATPGAPDHGFAPExtracellular ATP - ATP غیر سلولیMTT - MTT[Ca2+]i - [Ca2 +] iα,β-MeATP - α، β-MeATPα,β-methylene ATP - α، β-methylene ATPmaximal effect - اثر حداکثرCNS - دستگاه عصبی مرکزیAdult neural progenitor cells - سلول های پیشگیرانه عصبی بزرگسالانneural progenitor cell - سلول پیشرو عصبیcentral nervous system - سیستم عصبی مرکزیepidermal growth factor - عامل رشد اپیدرمیintracellular Ca2+ concentration - غلظت Ca2 + داخل سلولیfibroblast growth factor-2 - فیبروبلاست رشد فاکتور 2Musashi1 - مسعشی 1subventricular zone - منطقه فرعیwild-type - نوع وحشیGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازP2X7 receptors - گیرنده های P2X7
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب رفتاری
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چکیده انگلیسی
Neurogenesis requires the balance between the proliferation of newly formed progenitor cells and subsequent death of surplus cells. RT-PCR and immunocytochemistry demonstrated the presence of P2X7 receptor mRNA and immunoreactivity in cultured neural progenitor cells (NPCs) prepared from the adult mouse subventricular zone (SVZ). Whole-cell patch-clamp recordings showed a marked potentiation of the inward current responses both to ATP and the prototypic P2X7 receptor agonist dibenzoyl-ATP (Bz-ATP) at low Ca2+ and zero Mg2+ concentrations in the bath medium. The Bz-ATP-induced currents reversed their polarity near 0Â mV; in NPCs prepared from P2X7â/â mice, Bz-ATP failed to elicit membrane currents. The general P2X/P2Y receptor antagonist PPADS and the P2X7 selective antagonists Brilliant Blue G and A-438079 strongly depressed the effect of Bz-ATP. Long-lasting application of Bz-ATP induced an initial current, which slowly increased to a steady-state response. In combination with the determination of YO-PRO uptake, these experiments suggest the dilation of a receptor-channel and/or the recruitment of a dye-uptake pathway. Ca2+-imaging by means of Fura-2 revealed that in a Mg2+-deficient bath medium Bz-ATP causes [Ca2+]i transients fully depending on the presence of external Ca2+. The MTT test indicated a concentration-dependent decrease in cell viability by Bz-ATP treatment. Correspondingly, Bz-ATP led to an increase in active caspase 3 immunoreactivity, indicating a P2X7-controlled apoptosis. In acute SVZ brain slices of transgenic Tg(nestin/EGFP) mice, patch-clamp recordings identified P2X7 receptors at NPCs with pharmacological properties identical to those of their cultured counterparts. We suggest that the apoptotic/necrotic P2X7 receptors at NPCs may be of particular relevance during pathological conditions which lead to increased ATP release and thus could counterbalance the ensuing excessive cell proliferation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuropharmacology - Volume 73, October 2013, Pages 122-137
Journal: Neuropharmacology - Volume 73, October 2013, Pages 122-137
نویسندگان
Nanette Messemer, Christin Kunert, Marcus Grohmann, Helga Sobottka, Karen Nieber, Herbert Zimmermann, Heike Franke, Wolfgang Nörenberg, Isabelle Straub, Michael Schaefer, Thomas Riedel, Peter Illes, Patrizia Rubini,