Glycine release is regulated by metabotropic glutamate receptors sensitive to mGluR2/3 ligands and activated by N-acetylaspartylglutamate (NAAG)

► Depolarization of spinal cord nerve endings evokes glycine exocytosis. ► Glycine exocytosis is inhibited by Group II metabotropic glutamate receptors. ► N-acetylaspartylglutamate (NAAG) is a metabotropic glutamate receptor agonist of subtype 3.