Metabotropic glutamate receptor 5 antagonist 2-methyl-6-(phenylethynyl)pyridine (MPEP) microinfusions into the nucleus accumbens shell or ventral tegmental area attenuate the reinforcing effects of nicotine in rats

► Mesolimbic mGlu5 receptors play a role in nicotine reinforcement. ► Blockade of mGlu5 receptors in the NAcc attenuates nicotine intake. ► mGlu5 receptors in the VTA regulate both nicotine and food intake.