کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5827844 | 1558937 | 2014 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Subcellular distribution and early signalling events of P2X7 receptors from mouse cerebellar granule neurons
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کلمات کلیدی
PBSBBGBrilliant Blue GHEPESKN-62GFAPN-methyl-d-glucamineEGTANMDGBzATPDAPIA-4380794′6-diamidino-2-phenylindole - 4'6-diamidino-2-phenylindoleBSA - BSAadenosine 5′-triphosphate - آدنوزین 5'-تری فسفاتATP - آدنوزین تری فسفات یا ATPbovine serum albumin - آلبومین سرم گاوElectrophysiology - الکتروفیزیولوژیCNS - دستگاه عصبی مرکزیcentral nervous system - سیستم عصبی مرکزیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMicrofluorometry - میکرو فلوئورومتریcerebellar granule neurons - نورونهای گرانشی مخچهGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالP2X7 receptors - گیرنده های P2X7
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Subcellular distribution and early signalling events of P2X7 receptors from mouse cerebellar granule neurons Subcellular distribution and early signalling events of P2X7 receptors from mouse cerebellar granule neurons](/preview/png/5827844.png)
چکیده انگلیسی
The subcellular distribution and early signalling events of P2X7 receptors were studied in mouse cerebellar granule neurons. Whole-cell patch-clamp recordings evidenced inwardly directed non-desensitizing currents following adenosine 5â²-triphosphate (ATP; 600 µM) or 2â²-3â²-o-(4-benzoylbenzoyl)-adenosine 5â²-triphosphate (BzATP; 100 µM) administration to cells bathed in a medium with no-added divalent cations (Ca2+ and Mg2+). Nucleotide-activated currents were inhibited by superfusion of 2.5 mM Ca2+, 1.2 mM Mg2+ or 100 nM Brilliant Blue G (BBG), hence indicating the expression of ionotropic P2X7 receptors. Fura-2 calcium imaging showed [Ca2+]i elevations in response to ATP or BzATP at the somas and at a small number of axodendritic regions of granule neurons. Differential sensitivity of these [Ca2+]i increases to three different P2X7 receptor antagonists (100 nM BBG, 10 μM 4-[(2S)-2-[(5-isoquinolinylsulfonyl)methylamino]-3-oxo-3-(4-phenyl-1-piperazinyl)propyl] phenyl isoquinolinesulfonic acid ester, KN-62, and 1 μM 3-(5-(2,3-dichlorophenyl)-1H-tetrazol-1-yl)methyl pyridine hydrochloride hydrate, A-438079) revealed that P2X7 receptors are co-expressed with different P2Y receptors along the plasmalemma of granule neurons. Finally, experiments with the fluorescent dye YO-PRO-1 indicated that prolonged stimulation of P2X7 receptors does not lead to the opening of a membrane pore permeable to large cations. Altogether, our results emphasise the expression of functional P2X7 receptors at both the axodendritic and somatic levels in mouse cerebellar granule neurons, and favour the notion that P2X7 receptors might function in a subcellular localisation-specific manner: presynaptically, by controlling glutamate release, and on the cell somas, by supporting granule neuron survival against glutamate excytotoxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 744, 5 December 2014, Pages 190-202
Journal: European Journal of Pharmacology - Volume 744, 5 December 2014, Pages 190-202
نویسندگان
Jesús Sánchez-Nogueiro, Patricia MarÃn-GarcÃa, Diego Bustillo, Luis Alcides Olivos-Oré, MarÃa Teresa Miras-Portugal, Antonio R. Artalejo,