Role of interplay between IL-4 and IFN-γ in the in regulating M1 macrophage polarization induced by Nattectin

► The increase in the MHC class II expression induced by Nattectin, and CD49a are dependent on its lectin function. ► Nattectin induces MMP-9 production and endocytic capacity depends on lectin function. ► Nattectin induces M1 activation of macrophages. ► The influx of macrophages induced by Nattectin is totally dependent on IL-4 and IFN-γ. ► IL-4 is able to work as a coadjuvant with IFN-γ and IL-12 in the macrophage M1 polarization induced by Nattectin.