Isolation and characterization of LIGHT (TNFSF14) gene homologue in zebrafish (Danio rerio)

► We successfully isolated the cDNA of zebrafish LIGHT (zLIGHT) from zebrafish spleen. ► The recombinant protein SUMO-zsLIGHT was efficiently expressed in Escherichia coli BL21 (DE3). ► Our result proved the previous speculation that zebrafish LIGHT located on chr 3. ► Zebrafish may conduct as a model animal for further research on LIGHT.