The sphingosine-1-phosphate receptor-1 antagonist, W146, causes early and short-lasting peripheral blood lymphopenia in mice

► Treatment wth a S1P1 antagonist, W146, induces peripheral blood lymphopenia in mice. ► The pharmacokinetics of W146 explain its fast and short lasting lymphopenia. ► W146 also causes lung edema, due to the blockade of S1P1 receptor in endothelium. ► The lung edema and lymphopenia evoked by W146 show a similar time course. ► S1P1 agonists and antagonists cause the same in vivo effects with different kinetics.