MyD88-dependent toll-like receptor signaling is required for murine macrophages response to IS2

► TLR/MyD88 signaling pathway is responsible in IS2 β-glucan-mediated cellular response in RAW264.7 murine macrophages. ► IS2 β-glucan activates and leads to cytokine secretions of IL-6 and TNF-α in murine macrophages through TLR2 and TLR4. ► Phosphorylation of MAPKs and degradation of IκB-α was evident after stimulation with IS2 β-glucan.