Molecular cloning, expression, bioinformatics analysis and bioactivity characterization of TNF13B (BAFF) gene in bat (Vespertilio superans Thomas)

► This is the first report of BAFF gene cloned from chiroptera mammals. ► The deduced amino acid sequence was analyzed using bioinformatics methods. ► Protein was expressed in E. coli and purified by Ni column. ► Indirect immunofluorescence staining was conducted to determine receptor-binding activity. ► MTT analysis tested the bioactivity of BAFF to B cells.