کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5833994 1122636 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of the immunosuppressive activity of artesunate in vitro and in vivo
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Evaluation of the immunosuppressive activity of artesunate in vitro and in vivo
چکیده انگلیسی


- We examine the immunomodulatory effects of AST both in vitro and in vivo.
- AST has a relatively high immunosuppressive activity with low toxicity in vitro.
- AST could inhibit T cell-mediated immune response via promoting Tregs in vivo.
- AST might be considered as a safe putative candidate for immune-related diseases.

Artemisinin and its derivatives have been reported to have immunosuppressive activity in some laboratory studies. However, the detail of mechanism remains to be demonstrated. The objective of this study is to clarify the immunosuppressive activity of artesunate (AST), one kind of artemisinin derivatives, and to find its unexplored mode of action. In vitro, the proliferation of T lymphocytes and its cytotoxicity were measured by WST-1 and MTT assay. In vivo, the immunomodulatory effect of AST was evaluated in a mouse model of delayed type hypersensitivity reaction (DTH), which was based on a T cell-mediated immune response. The data displayed that AST had a relatively high immunosuppressive activity with low toxicity, and could inhibit T lymphocyte proliferation induced by mitogen and alloantigen. Meanwhile, topical administration of AST could suppress DTH response significantly. Moreover, AST could also increase the secretion of TFG-β, coupling with the striking enhance of NF-κB/p65 and Smad2/3 signaling. The promotion of CD4+CD25+ regulatory T cells (Tregs) was shown to be a possible mechanism involved in AST-mediated regulation. Taken together, these observations exhibit the potential of developing AST as a novel safe remedy for the treatment of T cell-mediated immune disorders.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 16, Issue 2, June 2013, Pages 306-312
نویسندگان
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