کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5834068 | 1122640 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of the Mac-1 and VLA-4 integrins, and concomitant Th2-cytokine production, in nitric oxide modulated eosinophil migration from bone marrow to lungs in allergic mice
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کلمات کلیدی
EotaxinVLA-4ICAM-1iNOSVCAM-1OVABALLFA-11400W - 1400 واتAllergic inflammation - التهاب آلرژیکOvalbumin - اوبلبومینvery late antigen-4 - خیلی زود antigen-4inducible nitric oxide synthase - سنتاز اکسید نیتریک القاییbronchoalveolar lavage - لارو برونکلو آلوئولارbone marrow - مغز استخوانintercellular adhesion molecule-1 - مولکول چسبندگی بین سلولی -1Mac-1 - مک 1Nitric oxide - نیتریک اکسید
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Although numerous studies demonstrate the participation of nitric oxide (NO) in various inflammatory diseases, the precise function of NO in allergic asthma remains unclear. We investigated whether iNOS inhibition could interfere with the kinetics of VLA-4 and Mac-1 expression and adhesion properties of bone marrow and peripheral blood eosinophils of sensitized mice after antigen exposure. Treatment of allergic mice with 1400W (iNOS inhibitor) increased the adhesion of bone marrow eosinophils to ICAM-1, but not blood eosinophils, at 24Â h and 48Â h after OVA-challenge. Conversely, adhesion of blood eosinophils from 1400W-treated mice to VCAM-1 diminished at 24Â h and was almost completely blocked at 48Â h. 1400W did not induce any change in the adhesion of bone marrow eosinophils to VCAM-1, at 24Â h, but cells collected 48Â h after challenge showed significantly lower adherence. Flow cytometry demonstrated that 1400W resulted in a significantly increased Mac-1 expression on bone marrow eosinophils at 24Â h, as compared to control mice. However, at 24Â h, 1400W significantly decreased Mac-1 and VLA-4 expressions on blood eosinophils. At 48Â h, the expressions of both Mac-1 and VLA-4 returned to previous levels. Results show a temporal effect of iNOS upon Mac-1 expression and function, the chief adhesion molecule involved in the eosinophil efflux from the bone marrow at 24Â h. In contrast, Mac-1 and VLA-4 were involved in eosinophil mobilization from blood to lungs at 48Â h after antigen challenge. Data suggest an important role of the Mac-1 and VLA-4 in the iNOS-modulated migration of eosinophils to the lungs of allergic mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 11, Issue 2, February 2011, Pages 204-211
Journal: International Immunopharmacology - Volume 11, Issue 2, February 2011, Pages 204-211
نویسندگان
Edmar Henrique Pelaquini, Lucila de Assis Figueiredo Guimarães, LetÃcia Regina Benetti, Luiz Gustavo Romani Fernandes, Wirla Maria da Silva Cunha Tamashiro, Nicola Conran, Heloisa Helena de Araujo Ferreira,