کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5834150 | 1122662 | 2012 | 4 صفحه PDF | دانلود رایگان |

BackgroundThe etiopathogenesis of Hashimoto's thyroiditis (HT) has not been clearly elucidated although the role of chronical inflammation and endothelial dysfunction has been established. The imbalance between pro- and anti-inflammatory cytokines may play a role in the etiology. The aim of the present study was to investigate whether cytokine gene polymorphisms are associated with HT, and to evaluate the relationship between genotypes and clinical/laboratory manifestation of HT.MethodsTumor necrosis factor α (TNFα) G-308A (rs 1800629), interleukin-6 (IL-6) G-174C (rs 1800795) and IL-10 G-1082A (rs 1800896) single nucleotide polymorphisms (SNPs) in DNA from peripheral blood leukocytes of 190 patients with HT and 231 healthy controls were investigated by real-time PCR combined with melting curve analysis using fluorescence-labeled hybridization probes.ResultsThere was no notable risk for HT afflicted by TNFα â 308, IL-6 â 174 and IL-10 â 1082 polymorphisms alone. However, carriers of variant alleles of both IL-10 â 1082 and TNFα â 308 polymorphisms had four-fold times higher risk for HT in comparison with non-carriers. Additionally, concomitant presence of both mutant IL-10 â 1082 A and IL-6 â 174 C alleles raised three-fold the HT risk.ConclusionOur results suggest that the combined effects of TNFα â 308, IL-6 â 174 and IL-10 â 1082 variant alleles may be more decisive to induce functional differences and modify the risk for HT.
⺠We investigated the possible relationship between cytokine polymorphisms and HT ⺠TNFα G-308A, IL-6 G-174C, IL-10 G-1082A polymorphisms were assayed by real-time PCR ⺠The TNFα â 308, IL-6 â 174, IL-10 â 1082 polymorphisms are not risk factors for HT alone ⺠The concomitant presence of mutant IL-10/TNFα, IL-10/IL-6 alleles rised risk for HT
Journal: International Immunopharmacology - Volume 12, Issue 4, April 2012, Pages 543-546