Effects of smoking regimens and test material format on the cytotoxicity of mainstream cigarette smoke

► Cytotoxicity among the test cigarettes were greater for WS compared to TPM. ► For TPM testing, cytotoxicity was decreased going from ISO regimen to HCI regimen. ► For WS, cytotoxicity was not consistent among three cigarettes between HCI and ISO. ► GVP was an important contributor to WS cytotoxicity.