کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5853672 | 1561795 | 2012 | 6 صفحه PDF | دانلود رایگان |

Plants as important source of natural active components with anticancer effects commonly are different in structure and biological properties. The pericarp of Pistacia atlantica sub kurdica with local name of Baneh, a rich source of active phytochemicals, contains noticeable amounts of polyphenolic compounds, flavonoids and anthocyanins. Therefore, the antiproliferative, apoptosis induction and cell cycle alterations of Baneh were evaluated in human colon carcinoma HT29 cells. The Baneh extract (0.7Â mg/ml) resulted in 50% growth inhibition similar to 500Â nM of Doxorubicin (Dox) in HT29 cells after 72Â h. The down-regulation of cyclin A protein by Baneh extract induced S phase delay in cell cycle progression of HT29 cells. Unlike the Baneh extract, Dox showed G2/M accumulation of HT29 cells which was associated with an increase in cyclin A and cyclin B1 protein expression. Furthermore, the induction of apoptosis following Baneh extract and Dox treatment in HT29 cells was confirmed by DNA fragmentation and translocation of phosphatidylserine. The morphological characteristics of apoptosis were also observed in HT29 cells exposed to the Baneh extract and Dox. These results suggest that due to the existence of bioactive components, methanolic extract of the Baneh has significant cytotoxic effects against human colon carcinoma HT29 cells.
⺠Baneh extract shows similar growth inhibition to Doxorubicin in HT29 cancer cells. ⺠Both Baneh extract and Dox induced apoptosis by translocation of phosphatidylserine. ⺠Doxorubicin induced G2/M arrest via increase in the cyclin A and cyclin B1 proteins. ⺠Unlike Dox, Baneh extract induced S phase delay via decrease in cyclin A protein. ⺠Baneh extract shows significant anticancer properties on cancer cell proliferation.
Journal: Food and Chemical Toxicology - Volume 50, Issues 3â4, MarchâApril 2012, Pages 1054-1059