کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5854167 | 1130885 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ethanol extract of Ocimum sanctum exerts anti-metastatic activity through inactivation of matrix metalloproteinase-9 and enhancement of anti-oxidant enzymes
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کلمات کلیدی
LLCMMPIEMEMMMPBCAMMP-9ECMPBSCATPMSXTTFBSBSA - BSADMSO - DMSOGSH-Px - GSH-PXH&E - H & EMTT - MTTOcimum sanctum - OcimumROS - ROSbovine serum albumin - آلبومین سرم گاوbicinchoninic acid - بیسینکنینیک اسیدDimethyl sulfoxide - دیمتیل سولفواکسیدSOD - سدfetal bovine serum - سرم جنین گاوSuperoxide dismutase - سوکسوکس دیسموتازAnti-oxidant - ضد اکسید کنندهPhosphate buffered saline - فسفات بافر شورphenazine methosulphate - فنازین متوسولفاتExtracellular matrix - ماتریکس خارج سلولیmatrix metalloproteinase - ماتریکس متالوپروتئینازMetastasis - متاستاز MMP inhibitors - مهار کننده های MMPHematoxylin and Eosin - هماتوکسیلین و ائوزینCatalase - کاتالازLewis lung carcinoma - کارسینوم ریه لوئیسglutathione peroxidase - گلوتاتیون پراکسیدازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ocimum sanctum has been known to possess various beneficial properties including anti-oxidative, anti-inflammatory and anti-cancer activities. In the present study, we investigated that ethanol extracts of O. sanctum (EEOS) had anti-metastatic activity through activation of anti-oxidative enzymes. EEOS exerted cytotoxicity against Lewis lung carcinoma (LLC) cells. Also, EEOS significantly inhibited cell adhesion and invasion as well as activities of matrix metalloproteinase-9 (MMP-9), but not MMP-2, indicating the important role of MMP-9 in anti-metastatic regulation of EEOS. In addition, EEOS significantly reduced the tumor nodule formation and lung weight in LLC-injected mice. Inhibitory effect of EEOS on metastasis was further confirmed by using hematoxylin and eosin (H&E) staining. Notably, we also found that EEOS enhanced activities of anti-oxidative enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in a concentration-dependent manner. Taken together, our findings support that EEOS can be a potent anti-metastatic candidate through inactivation of MMP-9 and enhancement of anti-oxidant enzymes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 48, Issue 6, June 2010, Pages 1478-1482
Journal: Food and Chemical Toxicology - Volume 48, Issue 6, June 2010, Pages 1478-1482
نویسندگان
Sun-Chae Kim, Venkataraman Magesh, Soo-Jin Jeong, Hyo-Jung Lee, Kyoo-Seok Ahn, Hyo-Jeong Lee, Eun-Ok Lee, Sun-Hyung Kim, Min-Ho Lee, Jung Hyo Kim, Sung-Hoon Kim,