کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5856583 1131978 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of EPA's endocrine screening battery and recommendations for further review
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Analysis of EPA's endocrine screening battery and recommendations for further review
چکیده انگلیسی


- EPA screens chemicals for the potential to interact with the endocrine system.
- Analyses reveal that many of the screening assays have poorly-defined performance criteria.
- Poorly-defined criteria will result in discarded data and wasted resources.
- EPA should review all screening assays prior to issuing further test orders.

EPA's Endocrine Disruptor Screening Program Tier 1 battery consists of eleven assays intended to identify the potential of a chemical to interact with the estrogen, androgen, thyroid, or steroidogenesis systems. We have collected control data from a subset of test order recipients from the first round of screening. The analysis undertaken herein demonstrates that the EPA should review all testing methods prior to issuing further test orders. Given the frequency with which certain performance criteria were violated, a primary focus of that review should consider adjustments to these standards to better reflect biological variability. A second focus should be to provide detailed, assay-specific direction on when results should be discarded; no clear guidance exists on the degree to which assays need to be re-run for failing to meet performance criteria. A third focus should be to identify permissible differences in study design and execution that have a large influence on endpoint variance. Experimental guidelines could then be re-defined such that endpoint variances are reduced and performance criteria are violated less frequently. It must be emphasized that because we were restricted to a subset (approximately half) of the control data, our analyses serve only as examples to underscore the importance of a detailed, rigorous, and comprehensive evaluation of the performance of the battery.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Toxicology and Pharmacology - Volume 72, Issue 3, August 2015, Pages 552-561
نویسندگان
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