Acute and subchronic toxicity of arprinocid in Sprague-Dawley rats

- Arprinocid is a moderately toxic substance.
- Arprinocid may have an affect on the hematopoietic function.
- Liver and kidney are possibly the main target organs of arprinocid.
- Females are more sensitive to arprinocid compared with males.
- The dietary NOAEL of arprinocid in rats was 25 ppm (approximately 1.7 mg/kg b.w.).

We subjected Sprague-Dawley rats to an acute and 13-week subchronic oral toxicity of arprinocid, a nucleoside analogue used as a coccidiostat, according to toxicological guidelines as part of its safety assessment. In the acute study, arprinocid was administered once by oral gavage to rats at doses ranging from 292.4 to 506.0 mg/kg b.w. The calculated LD50 was 442.9 mg/kg b.w. in males and 378.7 mg/kg b.w. in females. In the subchronic study, male and female rats were fed with diets supplemented with 0, 25, 187.5 or 500 ppm arprinocid for 13 weeks. Significantly lower body weights were noted in the 500 ppm group females. The mean body weights of the 500 ppm group females were 12.9% lower than that of the controls. Significant differences in haematological and biochemical parameters as well as organ weights were detected between the 500 and 187.5 ppm groups. Histopathological observations revealed that 500 and 187.5 ppm arprinocid could induce hepatic steatosis and focal hepatocellular necrosis. Slight protein cast in some renal tubules and tubular regeneration were observed in the high dose group of both genders. The dietary no-observed-adverse-effect level (NOAEL) of arprinocid in rats for 13 weeks is 25 ppm (approximately 1.7 mg/kg b.w./day).