کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5857936 | 1562154 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Supplementation with nanomolar concentrations of verbascoside during in vitro maturation improves embryo development by protecting the oocyte against oxidative stress: a large animal model study
ترجمه فارسی عنوان
تکثیر با غلظت نانو مولکولی ورباسکوزید در طی بلوغ انسانی، رشد جنین را با محافظت از تخمک در برابر استرس اکسیداتیو بهبود می بخشد: یک مطالعه مدل بزرگ حیوان
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
The effects of verbascoside (VB), added at nanomolar concentrations during in vitro maturation (IVM) of juvenile sheep oocytes, on in vitro embryo development and its mechanisms of action at the oocyte level were analyzed. Developmental rates, after IVM in the presence/absence of VB (1Â nM for 24Â h; 1Â nM for 2Â h; 10Â nM for 2Â h), were evaluated. The bioenergetic/oxidative status of oocytes matured after IVM in the presence/absence of 1Â nM VB for 24Â h was assessed by confocal analysis of mitochondria and reactive oxygen species (ROS), lipid peroxidation (LPO) assay, and quantitative PCR of bioenergy/redox-related genes. The addition of 1Â nM VB during 24Â h IVM significantly increased blastocyst formation and quality. Verbascoside reduced oocyte ROS and LPO and increased mitochondria/ROS colocalization while keeping mitochondria activity and gene expression unchanged. In conclusion, supplementation with nanomolar concentrations of VB during IVM, in the juvenile sheep model, promotes embryo development by protecting the oocyte against oxidative stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 65, October 2016, Pages 204-211
Journal: Reproductive Toxicology - Volume 65, October 2016, Pages 204-211
نویسندگان
Nicola Antonio Martino, Federica Ariu, Daniela Bebbere, Manuel Filioli Uranio, Adriana Chirico, Giuseppina Marzano, Anna Maria Sardanelli, Angela Cardinali, Fiorenza Minervini, Luisa Bogliolo, Maria Elena Dell'Aquila,