کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5858101 | 1562160 | 2016 | 14 صفحه PDF | دانلود رایگان |
- High Cr concentrations decreased boar sperm motility in vitro
- Cr affected mitochondrial activity, decreased the generation of ATP, and subsequently down-regulated protein tyrosine phosphorylation in the middle piece of boar sperm
- Cr may affected boar sperm motility by impairing protein tyrosine phosphorylation in the midpiece of sperm by blocking the cAMP/PKA pathway in boar sperm in vitro.
- This study is expected to provide a theoretical basis for the development of livestock industry and interesting clues for further study on reproductive toxicity.
Hexavalent chromium reportedly induces reproductive toxicity and further inhibits male fertility in mammals. In this study, we investigated the molecular mechanism by which hexavalent chromium affects motility signaling in boar spermatozoa in vitro. The results indicated that Cr(VI) decreased sperm motility, protein phosphorylation, mitochondrial membrane potential (ÎΨm) and metabolic enzyme activity starting at 4 μmol/mL following incubation for 1.5 h. Notably, all parameters were potently inhibited by 10 μmol/mL Cr, while supplementation with the dibutyryl-cAMP (dbcAMP) and the 3-isobutyl-1-methylxanthine (IBMX) prevented the inhibition of protein phosphorylation. Interestingly, high concentrations of Cr (>10 μmol/mL) increased the tyrosine phosphorylation of some high-molecular-weight proteins in the principle piece but decreased that in the middle piece associated with an extreme reduction of sperm motility. These results suggest that chromium affects boar sperm motility by impairing tyrosine phosphorylation in the midpiece of sperm by blocking the cAMP/PKA pathway in boar sperm in vitro.
Journal: Reproductive Toxicology - Volume 59, January 2016, Pages 66-79