کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5858612 | 1562177 | 2013 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Subchronic exposure to lead acetate inhibits spermatogenesis and downregulates the expression of Ddx3y in testis of mice
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
Male mice were given drinking water alone or containing 0.5, 1.0 and 1.5Â g/L Pb acetate for 60 days. After the treatment, the concentrations of Pb were determined in the serum and testis of mice by ICP-MS. The gene transcription of Usp9y, Ddx3y and Uty and their protein levels in the testis were examined by real-time PCR, Western blotting and immunohistochemistry. The sperm quality, spermatogenesis, and histological alteration of the testis and epididymis were observed by microscope. The probability of impregnating female mice by lead-exposed male was evaluated. It was shown that the concentrations of Pb in serum and testis of mice significantly increased in the groups exposed to Pb in a dose-dependent manner. The male fertility significantly decreased in the groups exposed to 1.0 and 1.5Â g/L Pb acetate. Moreover, exposure to Pb also inhibited spermatogenesis and sperm development, and significantly downregulated expressions of Ddx3y gene expression in testis of mice, but Usp9y and Uty expressions unaffected.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 42, December 2013, Pages 242-250
Journal: Reproductive Toxicology - Volume 42, December 2013, Pages 242-250
نویسندگان
Xiaoxu Wang, Man Wang, Wei Dong, Yachen Li, Xiaomei Zheng, Fengyuan Piao, Sheng Li,