Time-dependent variation in the biodistribution of C60 in rats determined by liquid chromatography-tandem mass spectrometry

► Biodistribution of C60 in rats after tail vein administration was examined using LC-MS/MS. ► C60 was found in various tissues including brain and the highest value was detected in the lungs. ► A time-dependent decrease in C60 concentrations was observed in all tissues, except spleen. ► A decreasing trend differed among tissues, which could be due to differences in accumulation level.