Sodium arsenite dependent protein expression analysis on human embryonic carcinoma (NCCIT) cell line

► We define the effect of NaAsO2 in early stages of cellular development. ► NaAsO2, in short-term downregulates PDI, PTPRF, HSP90 and VCP, affecting cellular growth or apoptosis. ► NaAsO2, in long-term upregulates B23/nucleophosmin, GRP78, ULK3, TLR6 and MAP3K7. ► Long-term NaAsO2 mainly affects the apoptotic and inflammatory pathways. ► Moreover, we find the non-lethal toxic stresses in NCCIT cells and EBs.