Reproductive toxicology of 2,4 dinitrophenol in boar sperm

The aim of this study was to evaluate the toxic effects of 2,4 dinitrophenol (DNP) on swine spermatozoa; particularly plasma membrane fluidity and integrity, mitochondrial function and motility, including oxidative stress parameters of lipid peroxidation and production of reactive oxygen species, when cooling to 17 °C during 24 to 96 h. We analyzed 22 ejaculates exposed to extender Beltsville Thawing Solution (BTS) (control) and in the same diluent concentrations of 0.01 μM (T1), 0.1 μM (T2), 1.0 μM (T3) and 10 μM (T4) of DNP. Shapiro Wilk test indicated the data was not normally distributed and means were compared using the Kruskal-Wallis test. Statistical analysis showed that DNP does not differ from the control, regardless of storage time, for any of the variables. Finally, quality parameters among the groups did not differ.