کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5861323 1133758 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of the ostreolysin A/pleurotolysin B pore-forming complex on intracellular Ca2 + activity in the vascular smooth muscle cell line A10
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Effect of the ostreolysin A/pleurotolysin B pore-forming complex on intracellular Ca2 + activity in the vascular smooth muscle cell line A10
چکیده انگلیسی
Ostreolysin A/pleurotolysin B (OlyA/PlyB) is a binary pore-forming protein complex that produces a rapid cardiorespiratory arrest. Increased tonus of the coronary vascular wall produced by OlyA/PlyB may lead to ischemia, arrhythmias, the hypoxic injury of cardiomyocytes and cardiotoxicity. We evaluated the effects of OlyA/PlyB in cultured vascular smooth muscle A10 cells. Fluorometric measurements using the Ca2 + indicator Fluo-4 AM and Fura-2 AM revealed that nanomolar concentrations of OlyA/PlyB increased the intracellular Ca2 + activity [Ca2 +]i in A10 cells. This effect was absent in a Ca2 +-free medium, indicating that OlyA/PlyB-induced [Ca2 +]i increase was dependent on Ca2 + influx into cells. The increase in [Ca2 +]i by OlyA/PlyB was partially prevented by: i) the calcium channel blockers verapamil and La3 +, ii) the inhibitor of the sodium-calcium exchanger (NCX) benzamil, and iii) the iso-osmotic replacement of NaCl by sucrose. The pre-treatment of cells with the Ca2 +-ATPase inhibitor thapsigargin reduced the [Ca2 +]i increase evoked by OlyA/PlyB, whereas the plasma membrane depolarization with high K+ in the medium did not prevent OlyA/PlyB-induced [Ca2 +]i. In summary, our data could suggest that the OlyA/PlyB-induced increase in [Ca2 +]i is due to an influx of Ca2 + through a variety of co-existing plasma membrane Ca2 +-permeable channels, Ca2 + entry through non-selective ion permeable pores formed de novo by OlyA/PlyB in the plasma membrane and calcium-induced intracellular Ca2 + release, altogether leading to disturbed Ca2 + homeostasis in A10 cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 29, Issue 8, December 2015, Pages 2015-2021
نویسندگان
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