کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5861534 1133761 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influence of physicochemical properties of silver nanoparticles on mast cell activation and degranulation
ترجمه فارسی عنوان
تأثیر خواص فیزیکی و شیمیایی نانوذرات نقره بر فعال شدن و انقراض سلولهای مشتانه
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی


- Silver nanoparticles induced mast cell degranulation.
- Degranulation was dependent on nanoparticle size, shape and surface coating.
- Scavenger receptor B1 is involved in the degranulation of mast cells.
- Ag+ dissolution did not contribute to mast cell degranulation.
- Silver nanoparticles may initiate or promote allergic immune responses.

Silver nanoparticles (AgNPs) are increasingly being incorporated into products for their antimicrobial properties. This has resulted in increased human exposures and the possibility of adverse health effects. Mast cells orchestrate allergic immune responses through degranulation and release of pre-formed mediators. Little data exists on understanding interactions of AgNPs with mast cells and the properties that influence activation and degranulation. Using bone marrow-derived mast cells and AgNPs of varying physicochemical properties we tested the hypothesis that AgNP physicochemical properties influence mast cell degranulation and osteopontin production. AgNPs evaluated included spherical 20 nm and 110 nm suspended in either polyvinylpyrrolidone (PVP) or citrate, Ag plates suspended in PVP of diameters between 40-60 nm or 100-130 nm, and Ag nanowires suspended in PVP with thicknesses <100 nm and length up to 2 μm. Mast cell responses were found to be dependent on the physicochemical properties of the AgNP. Further, we determined a role for scavenger receptor B1 in AgNP-induced mast cell responses. Mast cell degranulation was not dependent on AgNP dissolution but was prevented by tyrosine kinase inhibitor pretreatment. This study suggests that exposure to AgNPs may elicit adverse mast cell responses that could contribute to the initiation or exacerbation of allergic disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 29, Issue 1, February 2015, Pages 195-203
نویسندگان
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