کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5861536 1133761 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
ABCB1 and ABCC4 efflux transporters are involved in methyl parathion detoxification in ZFL cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
ABCB1 and ABCC4 efflux transporters are involved in methyl parathion detoxification in ZFL cells
چکیده انگلیسی


- The MXR mechanism is activated to detoxify from methyl parathion.
- The toxic effects of MP on ZFL cells were increased in the presence of the efflux transporter inhibitor.
- The combined exposure to MP and the inhibitor caused an increase in gene expression of P-gp1 (Abcb1) and MRP4 (Abcc4).

The multi-xenobiotics resistance (MXR) mechanisms are the first line of defense against toxic substances in aquatic organisms and present great importance in the adaptation related to contaminated environments. Methyl parathion (MP) is a widely used organophosphate pesticide, which has been associated to various toxic effects in organisms. In the present work, we studied the main genes related to efflux transporters in zebrafish liver (ZFL) cells exposed to MP with and without an inhibitor of ABC transporters (verapamil). The results concerning transporters activity showed that the MXR mechanism is activated to detoxify from methyl parathion. The toxic effects of MP on ZFL cells were increased in the presence of the efflux transporter inhibitor, once cell viability was significantly decreased in co-exposure experiments. The combined exposure to MP and the inhibitor caused an increase in gene expression of P-gp1 (Abcb1) and MRP4 (Abcc4), suggesting that these transporters isoforms are associated with MP efflux. In general, the expression of genes related to the antioxidant defense system (ADS) was significantly increased in ZFL cells co-exposed to MP and verapamil. These data provide useful insights for better understanding of MP detoxification mechanism in fish hepatocytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 29, Issue 1, February 2015, Pages 204-210
نویسندگان
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