کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5861773 | 1133765 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Investigating oxidative stress and inflammatory responses elicited by silver nanoparticles using high-throughput reporter genes in HepG2 cells: Effect of size, surface coating, and intracellular uptake
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کلمات کلیدی
PDIl-buthionine (S,R)-sulfoximineAgNO3interleukin 1BAP1BSOEC50GSHNrf2IC50PVPFBSDLS3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromide50% inhibitory concentration - 50٪ غلظت مهاریIL-1b - IL-1BMTT - MTTNFκB - NFKBAg NPs - NP NPo-Phenylenediamine - o-phenylenediamineROS - ROSopd - UPDAg+ - آگ +Cerium oxide - اکسید سریمCeO2 - اکسید سریم(IV)، سریک اکسیدOxidative stress - تنش اکسیداتیوfetal bovine serum - سرم جنین گاوpolydispersity index - شاخص پلییدریزاییinductively coupled plasma mass spectroscopy - طیف سنجی جرم پلاسما به صورت القاییICP-MS - طیفسنجی جرمی پلاسمای جفتشده القاییantioxidant response element - عنصر پاسخ آنتی اکسیدانnuclear factor (erythroid-derived 2)-like 2 - فاکتور هسته ای (erythroid-derived 2) -like 2nuclear factor kappa B - فاکتور هسته ای کاپا BNanoparticles - نانوذراتSilver nanoparticles - نانوذرات نقرهSilver - نقرهsilver nitrate - نیترات نقرهhalf maximal effective concentration - نیمه حداکثر غلظت موثرARE - هستندHydroquinone - هیدروکینونStress response - پاسخ به استرسDynamic Light Scattering - پراکندگی نور دینامیکیactivator protein 1 - پروتئین فعال کننده 1polyvinylpyrrolidone - پلی وینیل پیرولیدونReporter genes - ژن خبرنگارGlutathione - گلوتاتیونReactive oxygen species - گونههای فعال اکسیژنSilver ion - یون نقره ای
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Silver nanoparticles (Ag NP) have been shown to generate reactive oxygen species; however, the association between physicochemical characteristics of nanoparticles and cellular stress responses elicited by exposure has not been elucidated. Here, we examined three key stress-responsive pathways activated by Nrf-2/ARE, NFκB, and AP1 during exposure to Ag NP of two distinct sizes (10 and 75 nm) and coatings (citrate and polyvinylpyrrolidone), as well as silver nitrate (AgNO3), and CeO2 nanoparticles. The in vitro assays assessed the cellular response in a battery of stable luciferase-reporter HepG2 cell lines. We further assessed the impact of Ag NP and AgNO3 exposure on cellular redox status by measuring glutathione depletion. Lastly, we determined intracellular Ag concentration by inductively coupled plasma mass spectroscopy (ICP-MS) and re-analyzed reporter-gene data using these values to estimate the relative potencies of the Ag NPs and AgNO3. Our results show activation of all three stress response pathways, with Nrf-2/ARE displaying the strongest response elicited by each Ag NP and AgNO3 evaluated here. The smaller (10-nm) Ag NPs were more potent than the larger (75-nm) Ag NPs in each stress-response pathway, and citrate-coated Ag NPs had higher intracellular silver concentrations compared with both PVP-coated Ag NP and AgNO3. The cellular stress response profiles after Ag NP exposure were similar to that of AgNO3, suggesting that the oxidative stress and inflammatory effects of Ag NP are likely due to the cytotoxicity of silver ions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 27, Issue 6, September 2013, Pages 2013-2021
Journal: Toxicology in Vitro - Volume 27, Issue 6, September 2013, Pages 2013-2021
نویسندگان
Raju Y. Prasad, John K. McGee, Micaela G. Killius, Danielle A. Suarez, Carl F. Blackman, David M. DeMarini, Steven O. Simmons,