کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5861931 | 1133769 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro exposure of tobacco specific nitrosamines decreases the rat lung phospholipids by enhanced phospholipase A2 activity
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کلمات کلیدی
EGTAFFADPPCLPCNNKDPPGBSA - BSADMSO - DMSObovine serum albumin - آلبومین سرم گاوEDTA - اتیلن دی آمین تترا استیک اسید Ethylene diamine tetra acetic acid - اتیلن دیامین تترا استیک اسیدethylene glycol tetraacetic acid - اتیلن گلیکول تتراستیک اسیدFree fatty acid - اسید چرب آزادdimethyl sulphoxide - دی متیل سولفوکسیدdipalmitoylphosphatidylcholine - دیپالمیتویف فسفاتیدیل کولینDipalmitoylphosphatidylglycerol - دیپالمیتویف فسفاتیدیل گلیسرولLysophosphatidylcholine - لیزوفسفاتیدیل کولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Tobacco-specific nitrosamines (TSNA) have implications in the pathogenesis of various lung diseases and conditions are prevalent even in non-smokers. N-nitrosonornicotine (NNN) and 4-(methyl nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) are potent pulmonary carcinogens present in tobacco product and are mainly responsible for lung cancer. TSNA reacts with pulmonary surfactants, and alters the surfactant phospholipid. The present study was undertaken to investigate the in vitro exposure of rat lung tissue slices to NNK or NNN and to monitor the phospholipid alteration by [32P]orthophosphate labeling. Phospholipid content decreased significantly in the presence of either NNK or NNN with concentration and time dependent manner. Phosphatidylcholine (PC) is the main phospholipid of lung and significant reduction was observed in PC â¼61%, followed by phosphatidylglycerol (PG) with 100 μM of NNK, whereas NNN treated tissues showed a reduction in phosphatidylserine (PS) â¼60% and PC at 250 μM concentration. The phospholipase A2 assays and expression studies reveal that both compounds enhanced phospholipid hydrolysis, thereby reducing the phospholipid content. Collectively, our data demonstrated that both NNK and NNN significantly influenced the surfactant phospholipid level by enhanced phospholipase A2 activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 28, Issue 6, September 2014, Pages 1097-1105
Journal: Toxicology in Vitro - Volume 28, Issue 6, September 2014, Pages 1097-1105
نویسندگان
Panneerselvam Vijayaraj, Chinnarasu Sivaprakasam, Lakshmanaperumal Vishnu Varthini, Mary Sarkar, Vasanthi Nachiappan,