Mono-(2-ethylhexyl) phthalate affects the steroidogenesis in rat Leydig cells through provoking ROS perturbation

► We explored the effect of MEHP on androgen production of rat Leydig cells. ► Low levels of MEHP increased androgen production. ► High levels of MEHP inhibited androgen production. ► In ALCs, MEHP affected androgen production through provoking ROS perturbation.