Non-invasive monitoring of cytotoxicity based on kinetic changes of cellular autofluorescence

► We use temporal profiles of cellular autofluorescence intensities as toxicity assay. ► Toxicity monitoring is fast, rapid on-line, simple and non-destructive. ► The assay responds accordingly to concentration and mode of action of the toxins. ► We proposed a bi-phased time-dose-response model to fit the AF profiles. ► The model allows quantitative analysis of toxicity of compounds.