Stobadine attenuates impairment of an intestinal barrier model caused by 4-hydroxynonenal

► Establishment of an in vitro intestinal barrier model for studying lipid peroxidation. ► Revealing 4-hydroxynonenal as a causative factor of intestinal barrier impairment. ► Prevention of HNE damaging effects by hydrophilic or lipophilic form of stobadine. ► Stobadine dipalmitate prevented HNE toxicity and reduced barrier permeability.