کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5890589 1153255 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Original Full Length ArticleInduced periosteum a complex cellular scaffold for the treatment of large bone defects
ترجمه فارسی عنوان
پریستعمار کامل ماده طولی اصلی داربست سلولی پیچیده برای درمان نقایص استخوانی بزرگ است
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
چکیده انگلیسی


- The induced membrane and periosteum shared strong architectural similarities, vascular features and growth factor expression.
- The induced membrane was a rich source of MSCs as shown by functional MSC trilineage assays.
- Induced membrane had an increased number of cells with a pericyte phenotype suggesting more active vessel formation and/or maturation.
- The relative abundance of SDF-1 transcript was greater in expanded cells from membrane, suggesting active recruitment of regenerative cells.
- The induced membrane technique results in formation of a neo-periosteum rich in cells and molecules key to bone regeneration.

ObjectiveSurgically induced periosteal membrane holds great potential for the treatment of large bone defects representing a simple alternative to combinations of exogenous stem cells, scaffolds and growth factors. The purpose of this study was to explore the biological basis for this novel regenerative medicine strategy in man.MethodsEight patients with critical size defects were treated with the induced membrane (IM) technique. After membrane formation 1 cm2 biopsy was taken together with matched, healthy diaphyseal periosteum (P) for comparative analysis. Morphological characteristics, cell composition and growth factor expression were compared. Functional and molecular evaluation of mesenchymal stromal cell (MSC) activity was performed.ResultsBoth tissues shared similar morphology although IM was significantly thicker than P (p = 0.032). The frequency of lymphocytes, pericytes (CD45−CD34−CD146+) and cells expressing markers consistent with bone marrow MSCs (CD45−/lowCD271bright) were 31. 3 and 15.5-fold higher respectively in IM (all p = 0.043). IM contained 3-fold more cells per gramme of tissue with a similar proportion of endothelial cells (CD45−CD31+). Expressed bone morphogenic protein 2, vascular endothelial growth factor and stromal derived factor 1 (SDF-1) are key tissue regeneration mediators. Adherent expanded cells from both tissues had molecular profiles similar to bone marrow MSCs but cells from IM expressed greater than 2 fold relative abundance of SDF-1transcript compared to P (p = 0.043).ConclusionThe IM is a thick, vascularised structure that resembles periosteum with a cellular composition and molecular profile facilitating large defect repair and therefore may be described as an “induced-periosteum”. This tissue offers a powerful example of in situ tissue engineering.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 57, Issue 2, December 2013, Pages 484-492
نویسندگان
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