کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5890840 1153261 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lentiviral gene transfer of TCIRG1 into peripheral blood CD34+ cells restores osteoclast function in infantile malignant osteopetrosis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Lentiviral gene transfer of TCIRG1 into peripheral blood CD34+ cells restores osteoclast function in infantile malignant osteopetrosis
چکیده انگلیسی


- TGIRG1 was expressed with a lentiviral vector in CD34+ cells from 5 IMO patients.
- Gene corrected IMO CD34+ cells were successfully differentiated to osteoclasts.
- Corrected IMO osteoclasts mediated increased release of calcium and CTX-1 on bone.
- Resorption of corrected IMO osteoclasts was 70-80% of that of control osteoclasts.
- Transduced IMO CD34+ cells successfully engrafted in NSG-mice.

Infantile malignant osteopetrosis (IMO) is a rare, lethal, autosomal recessive disorder characterized by non-functional osteoclasts. More than 50% of the patients have mutations in the TCIRG1 gene, encoding for a subunit of the osteoclast proton pump. The aim of this study was to restore the resorptive function of IMO osteoclasts by lentiviral mediated gene transfer of the TCIRG1 cDNA. CD34+ cells from peripheral blood of five IMO patients and from normal cord blood were transduced with lentiviral vectors expressing TCIRG1 and GFP under a SFFV promoter, expanded in culture and differentiated on bone slices to mature osteoclasts. qPCR analysis and western blot revealed increased mRNA and protein levels of TCIRG1, comparable to controls. Vector corrected IMO osteoclasts generated increased release of Ca2 + and bone degradation product CTX-I into the media as well as increased formation of resorption pits in the bone slices, while non-corrected IMO osteoclasts failed to resorb bone. Resorption was approximately 70-80% of that of osteoclasts generated from cord blood. Furthermore, transduced CD34+ cells successfully engrafted in NSG-mice. In conclusion we provide the first evidence of lentiviral-mediated correction of a human genetic disease affecting the osteoclastic lineage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 57, Issue 1, November 2013, Pages 1-9
نویسندگان
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