کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5891154 1153265 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Steering the osteoclast through the demineralization-collagenolysis balance
ترجمه فارسی عنوان
استئوس کلست را از طریق ترشح کمینالیزاسیون-کلاژنولیز، هدایت کنید
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
چکیده انگلیسی
First we observed that when culturing osteoclasts on bone slices, excavations appeared either as round pits containing demineralized collagen, or as elongated trenches without demineralized collagen. This suggests that round pits are generated when collagen degradation is slower than demineralization, and trenches when collagen degradation is as fast as demineralization. Next we treated the osteoclasts with a low dose of a carbonic anhydrase inhibitor to slightly decrease the rate of demineralization, thereby allowing collagen degradation to proceed as fast as demineralization. This resulted in about a two-fold increase of the proportion of trenches, thus supporting our hypothesis. The same result was obtained if facilitating collagen degradation by pre-treating the bone slices with NaOCl. In contrast, when decreasing the rate of collagenolysis vs. demineralization by the addition of a cathepsin K specific inhibitor, the proportion of trenches fell close to 0%, and furthermore the round pits became almost half as deep. These observations lead to a model where the osteoclast resorption route starts perpendicularly to the bone surface, forming a pit, and continues parallel to the bone surface, forming a trench. Importantly, we show that the progress of the osteoclast along this route depends on the balance between the rate of collagenolysis and demineralization. We propose that the osteocytes and bone lining cells surrounding the osteoclast may act on this balance to steer the osteoclast resorptive activity in order to give the excavations a specific shape.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 56, Issue 1, September 2013, Pages 191-198
نویسندگان
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