کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5901170 | 1568908 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Aspp2 negatively regulates body growth but not developmental timing by modulating IRS signaling in zebrafish embryos
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کلمات کلیدی
ANKSH3eGFPHEKIGFsERKhpfIRSenhanced GFPIGF-IR5-bromo-2′-deoxyuridine - 5-bromo-2'-deoxyuridineAnkyrin repeats - آنکرین تکرار می شودAcridine orange - آکاردین نارنجیAkt/PKB - آکت / PKBAmino acids - اسید آمینه یا آمینو اسیدinsulin receptor substrate - انسولین بستر گیرندهBrdU - بروموداکسی اوریدینhour post fertilization - ساعت پس از لقاحinsulin-like growth factors - عوامل رشد انسولین مانندSrc homology 3 - همبستگی Src 3protein kinase B - پروتئین کیناز Bhuman embryonic kidney - کلیه جنین انسانextracellular signal-regulated kinases - کیناز های تنظیم شده سیگنال خارج سلولیIGF-I receptor - گیرنده IGF-I
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Aspp2 negatively regulates body growth but not developmental timing by modulating IRS signaling in zebrafish embryos Aspp2 negatively regulates body growth but not developmental timing by modulating IRS signaling in zebrafish embryos](/preview/png/5901170.png)
چکیده انگلیسی
The growth and developmental rate of developing embryos and fetus are tightly controlled and coordinated to maintain proper body shape and size. The insulin receptor substrate (IRS) proteins, key intracellular transducers of insulin and insulin-like growth factor signaling, play essential roles in the regulation of growth and development. A short isoform of apoptosis-stimulating protein of p53 2 (ASPP2) was recently identified as a binding partner of IRS-1 and IRS-2 in mammalian cells in vitro. However, it is unclear whether ASPP2 plays any role in vertebrate embryonic growth and development. Here, we show that zebrafish Aspp2a and Aspp2b negatively regulate embryonic growth without affecting developmental rate. Human ASPP2 had similar effects on body growth in zebrafish embryos. Aspp2a and 2b inhibit Akt signaling. This inhibition was reversed by coinjection of myr-Akt1, a constitutively active form of Akt1. Zebrafish Aspp2a and Aspp2b physically bound with Irs-1, and the growth inhibitory effects of ASPP2/Aspp2 depend on the presence of their ankyrin repeats and SH3 domains. These findings uncover a novel role of Aspp2 in regulating vertebrate embryonic growth.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 197, 1 February 2014, Pages 82-91
Journal: General and Comparative Endocrinology - Volume 197, 1 February 2014, Pages 82-91
نویسندگان
Chengdong Liu, Jing Luan, Yan Bai, Yun Li, Ling Lu, Yunzhang Liu, Fumihiko Hakuno, Shin-Ichiro Takahashi, Cunming Duan, Jianfeng Zhou,