کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5904648 | 1158050 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Silk protein hydrolysate increases glucose uptake through up-regulation of GLUT 4 and reduces the expression of leptin in 3T3-L1 fibroblast
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کلمات کلیدی
PPAR-γIBMXDMEMFBSSREBP-1FASGLUT2-DG - 2 DG3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoMTT - MTTAdipogenesis - آدیپوژنزfatty acid synthase - اسید چرب سنتازisobutylmethylxanthine - ایزوبویل methylxanthinetriglyceride - تریگلیسریدGlucose transporter - حمل و نقل گلوکزfetal bovine serum - سرم جنین گاوLeptin - لپتین sterol regulatory element binding protein - پروتئین اتصال دهنده عصاره استرولPioglitazone - گلوتازونPeroxisome proliferator-activated receptor-γ - گیرنده پروتئینی فعال پروکسیوم - γ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The purpose of our research was to test the hypothesis that silk protein hydrolysate increases glucose uptake in cultured murine embryonic fibroblasts. Insulin sensitizing activity was observed in a cell-based glucose uptake assay using 3T3-L1 embryonic fibroblasts. The treatment of 1 mg/mL of silk peptide E5K6 plus 0.2 nM insulin was associated with a significant increase in glucose uptake (124.0% ± 2.5%) compared to treatment with 0.2 nM insulin alone. When the 3T3-L1 cells were induced to differentiate into fibroblasts, fat droplets formed inside the cells. Silk peptide E5K6 reduced the formation of fat droplets at the 1-mg/mL dosage (86.1% ± 2.5%) when compared to the control (100.0% ± 5.8%). A 1 mg/mL dose of silk peptide E5K6 significantly increased GLUT 4 expression (131.5% ± 4.0%). The treatment of 1 mg/mL of silk peptide E5K6 did not present any changes for adipogenic expressed genes, but leptin expression was significantly increased by silk peptide E5K6 supplementation (175.9% ± 11.1%). From these results, silk peptide E5K6 increased glucose uptake via up-regulation of GLUT 4 and decreased fat accumulation via the up-regulation of leptin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nutrition Research - Volume 31, Issue 12, December 2011, Pages 937-943
Journal: Nutrition Research - Volume 31, Issue 12, December 2011, Pages 937-943
نویسندگان
Hyun-Sun Lee, Hyun Jung Lee, Hyung Joo Suh,