کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5907639 | 1569919 | 2008 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular and functional characterization of a tandem-repeat galectin from the freshwater snail Biomphalaria glabrata, intermediate host of the human blood fluke Schistosoma mansoni
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کلمات کلیدی
RBCLacdiNAcN-acetyl-lactosamineLDNBGEGalNAcLacNAcPRRCRDGlcNAcGlcLACDICFBScDNA - cDNAComplementary DNA - DNA تکمیلیN-Acetyl-galactosamine - N-استیل گالاکتوزامینN-Acetyl-Glucosamine - N-استیل گلوکوزامینamino acid - آمینو اسیدEDTA - اتیلن دی آمین تترا استیک اسید EST - استInnate immunity - ایمنی ذاتیTrehalose - ترهالوز rapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدExpressed Sequence Tag - تکرار اظهار نظرHIS - خودcarbohydrate recognition domain - دامنه شناخت کربوهیدراتTRE - سهLactose - لاکتوزLectin - لکتینMannose - مانوزMan - مردRace - مسابقهHemocyte - هموسیتhistidine - هیستیدینdifferential interference contrast - کنتراست تداخل دیفرانسیلGal - گالGalactose - گالاکتوزمی red blood cell - گلبول قرمز، اریتروسیتGlucose - گلوکزPRR, Pattern recognition receptor - گیرنده های شناسایی الگو
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular and functional characterization of a tandem-repeat galectin from the freshwater snail Biomphalaria glabrata, intermediate host of the human blood fluke Schistosoma mansoni Molecular and functional characterization of a tandem-repeat galectin from the freshwater snail Biomphalaria glabrata, intermediate host of the human blood fluke Schistosoma mansoni](/preview/png/5907639.png)
چکیده انگلیسی
In the present study, a tandem-repeat type galectin was characterized from an embryonic cell line (Bge) and circulating hemocytes of the snail Biomphalaria glabrata, intermediate host of the human blood fluke Schistosoma mansoni. The predicted B. glabrata galectin (BgGal) protein of 32 kDa possessed 2 carbohydrate recognition domains, each displaying 6 of 8 conserved amino acids involved in galactoside-binding activity. A recombinant BgGal (rBgGal) demonstrated hemagglutinating activity against rabbit erythrocytes, which was specifically inhibited by galactose-containing sugars (lacNAc/lac > galNAc/gal). Although native galectin was immunolocalized in the cytoplasm of Bge cells and the plasma membrane of a subset of snail hemocytes (60%), it was not detected in cell-free plasma by Western blot analysis. The findings that rBgGal selectively recognizes the schistosome-related sugar, lacNAc, and strongly binds to hemocytes and the tegument of S. mansoni sporocysts in a sugar-inhibitable fashion suggest that hemocyte-bound galectin may be serving as a pattern recognition receptor for this, or other pathogens possessing appropriate sugar ligands. Based on molecular and functional features, BgGal represents an authentic galectin, the first to be fully characterized in the medically-important molluscan Class Gastropoda.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 411, Issues 1â2, 31 March 2008, Pages 46-58
Journal: Gene - Volume 411, Issues 1â2, 31 March 2008, Pages 46-58
نویسندگان
Timothy P. Yoshino, Nathalie Dinguirard, John Kunert, Cornelius H. Hokke,