Genomic characterization of EmsB microsatellite loci in Echinococcus multilocularis

- EmsB microsatellite copies and position in the Echinococcus multilocularis genome.
- 40 copies of EmsB highlighted by in silico analysis with polymorphic repetitions.
- Localization of EmsB on the chromosome 5 of the parasite.
- Tandemly repeated structure conserved between E. multilocularis and E. granulosus.
- Peak heights on EmsB profiles related to microsatellite copy number in the genome.

EmsB is a molecular marker applied to Echinococcus multilocularis genotyping studies. This marker has largely been used to investigate the epidemiology of the parasite in different endemic foci. The present study has lifted the veil on the genetic structure of this microsatellite. By in silico analysis on the E. multilocularis genome the microsatellite was described in about 40 copies on the chromosome 5 of the parasite. Similar structure was found in the relative parasite Echinococcus granulosus, where the microsatellite was firstly described. The present study completes the first investigations made on the EmsB microsatellite origins and confirms the reliability of this highly discriminant molecular marker.