Molecular characterization of the NSP4 gene of human group A rotavirus strains circulating in Tunisia from 2006 to 2008

► NSP4 genotypes detected among Tunisian rotavirus strains were E1 (68%) and E2 (32%). ► Genotype E1 was associated with G1P[8], G3P[6], G3P[8], G4P[6] and G4P[8] samples. ► Genotype E2 was associated with G2P[4], G2P[6] and G6P[9]. ► Tunisian rotavirus isolates clustered into two main human NSP4 lineages.