کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5914669 | 1162747 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The 1.9Â Ã
crystal structure of Prp20p from Saccharomyces cerevisiae and its binding properties to Gsp1p and histones
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کلمات کلیدی
MADmultiple-wavelength anomalous diffractionregulator of chromosome condensation 1DMABRCC1GEFNLSNPCRLDGSTRMSDIPTGisopropyl β-D-1-thiogalactopyranoside - ایزوپروپیل β-D-1-thiogalactopyranosideMolecular dynamics - دینامیک ملکولی یا پویایی مولکولیnuclear localization signal - سیگنال محلی سازی هسته ایRån - شتمGAP - شکافguanine nucleotide exchange factor - فاکتور تبادل نوکلئوتید گوانینnuclear pore complex - مجتمع مخرب هسته ایroot mean square deviation - میانگین انحراف مربع ریشهHistone - هیستونGTPase-activating protein - پروتئین فعال GTPaseglutathione S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
Prp20p is the homolog of mammalian RCC1 (regulator of chromosome condensation 1) in Saccharomyces cerevisiae, which acts as the guanine nucleotide exchange factor (GEF) for Gsp1p (yeast Ran). Prp20p plays multiple roles in mRNA metabolism, nucleocytoplasmic transport and mitosis regulation. Prp20p also functions as a linker between chromatin and nuclear pore complex (NPC) which regulates the NPC-mediated boundary activity (BA). Prp20p contains an N-terminal nuclear localization signal (NLS) and a typical RCC1-like domain (RLD). Here we present the 1.9Â Ã
crystal structure of the RCC1-like domain of Prp20p, which exhibits a classical seven-bladed β-propeller. We also proved that the additional β-wedge in Prp20p is essential for the interaction between Prp20p and Gsp1p. Based on this structure, we built a complex model of Prp20p and Gsp1p which was optimized by molecular dynamics (MD) simulations. Our model reveals that Prp20p and RCC1 share similar Ran GTPase binding mode. In addition, we also studied the histone-binding property of Prp20p in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 174, Issue 1, April 2011, Pages 213-222
Journal: Journal of Structural Biology - Volume 174, Issue 1, April 2011, Pages 213-222
نویسندگان
Fangming Wu, Yiwei Liu, Zhiqiang Zhu, Hongda Huang, Bo Ding, Jihui Wu, Yunyu Shi,