کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5916212 | 1163375 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
EhGEF2, a Dbl-RhoGEF from Entamoeba histolytica has atypical biochemical properties and participates in essential cellular processes
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کلمات کلیدی
ARMGEFRho GTPasesEntamoeba histolytica - Entamoeba هیستولیتیکاErythrophagocytosis - erythrophagocytosisarmadillo - آرامیدوphosphatidic acid - اسید فسفاتیدیکProliferation - ترویجguanine nucleotide exchange factor - فاکتور تبادل نوکلئوتید گوانینDbl homology - همبستگی DblPleckstrin Homology - همخوانی PleckstrinChemotaxis - کموتاکسی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Dbl proteins are a family of factors that exchange the guanine nucleotide which promote the activation of Rho small GTPases. This paper reports the molecular, structural, biochemical and functional characterization of EhGEF2, a new member of the Dbl family. EhGEF2 is the second GEF studied in parasites and in the protozoan Entamoeba histolytica, and it is also the first member of the Dbl family that was found to have Arm repeats. The catalytic domain (DH) of EhGEF2 has the conserved residues T421, N590 and E591, which are important for the activation of the GTPases. Biochemical studies on EhGEF2 showed that it could activate in vitro the amoebic GTPases EhRacA, EhRacB, EhRacC, EhRacD, EhRacG, EhRacH and EhCdc42, being EhRacG its main target. It was found that the DH domain binds specifically phosphatidic acid (PA); docking and lipid dot blot studies indicated that this binding does not interfere with the contact surface of EhRacG. Functional studies showed that both the Arm repeats and the catalytic domain of EhGEF2 participate in its localization at the amoebic membrane. Expression of a negative dominant version of EhGEF2 protein in E. histolytica provoked a 30% decrease in its ability to phagocyte human erythrocytes as well as severe effects on both the proliferation and the cellular chemotaxis which suggest that EhGEF2 participates in these cellular processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 151, Issue 1, January 2007, Pages 70-80
Journal: Molecular and Biochemical Parasitology - Volume 151, Issue 1, January 2007, Pages 70-80
نویسندگان
Claudia Haydée González De la Rosa, Luis Enrique Arias-Romero, Ma de Jesús Almaraz-Barrera, Rosaura Hernandez-Rivas, Alejandro Sosa-Peinado, Arturo Rojo-DomÃnguez, Martha Robles-Flores, Miguel Vargas,