Zwitterionic betaine-cholesterol system: Effects of sonication duration and aging on vesicles stability

- Vesicles (42-179 nm) formed from zwitterionic betaine surfactant/cholesterol system.
- At Ctot = 65 mM with 3:1 mol% of betaine/cholesterol the vesicles are stable for 8 months.
- 20-25 mol% mixing ratio of cholesterol minimized the vesicles size diameter.
- Long sonication with aging effects reduces vesicles size within a period of 6 weeks.